Solubilization and characterization of Herpesvirus saimiri-induced membrane antigens

Antibody patterns to Herpesvirus saimiri-induced antigens in owl monkeys.

Antibody-response patterns to 3 major groups of Herpesvirus saimiri (HVS)associated antigens [early antigens (EA), late antigens (LA), membrane antigens (MA)] in 10 owl monkeys infected with HVS were related to the clinical course of HVS-induced disease. Results are also presented which show that EA is produced 4-8 hours earlier than LA in HVSinfected cells providing further evidence that these...

Preventive vaccination against Herpesvirus saimiri-induced neoplasia.

In this paper we discuss the use of Herpesvirus saimiri as a model for the development of vaccines against herpesvirus-induced neoplasia in primates. Attempts at protection against the oncogenicity of H. saimiri have centered on the inactivation of virus by heat and formalin, the production of temperature-sensitive H. saimiri mutants, and the attenuation of the virus. Each of these approaches h...

Characterization of proteins induced by Herpesvirus saimiri: comparative immunoprecipitation and analysis of glycosylation.

Immediate-early transcription of Herpesvirus saimiri.

Transcription of Herpesvirus saimiri was characterized during the initial phases of productive infection by Northern blot analyses and hybridizations of radioactive cDNA with cloned fragments of virion L-DNA. Under conditions of immediate-early transcription, e.g., blocking of viral protein synthesis by cycloheximide, a single cytoplasmic polyadenylated viral RNA of 2.7 kilobases was found in i...

Isolation and characterization of monoclonal antibodies to structural and nonstructural herpesvirus saimiri proteins.

An improved screening procedure was applied to identify hybridomas secreting antibodies to herpesvirus saimiri-specified polypeptides among the products of fusions between SP2/0 myeloma cells and spleen cells from mice immunized with purified virus particles or virus-specific DNA-binding proteins. Twenty-four monoclonal antibodies were isolated with specificities for 13 different virus-specifie...